Caught by a hair: Quick, new identification of hair may help crime fighters

Lily Huang crushes up the human hair prior to testing. Credit: Image courtesy of Queen's University

Crime fighters could have a new tool at their disposal following promising research by Queen's professor Diane Beauchemin.

Dr. Beauchemin (Chemistry) and student Lily Huang (MSc'15) have developed a cutting-edge technique to identify human hair. Their test is quicker than DNA analysis techniques currently used by law enforcement. Early sample testing at Queen's produced a 100 per cent success rate.

"My first paper and foray into forensic chemistry was developing a method of identifying paint that could help solve hit and run cases," explains Dr. Beauchemin. "Last year, Lily wanted to research hair analysis, so I started working in that area."

Blood samples are often used to identify gender and ethnicity, but blood can deteriorate quickly and can easily be contaminated. Hair, on the other hand, is very stable. Elements in hair originate from sweat secretions that alter with diet, ethnicity, gender, the environment and working conditions.

Dr. Beauchemin's process takes 85 seconds to complete and involves grinding up the hair, burning it and then analyzing the vapour that is produced.

"Our analysis process is very robust and can be used universally," says Ms. Huang. "One of our samples even included dyed hair and the test was 100 per cent accurate. The test was able to distinguish East Asians, Caucasians and South Asians."

Dr. Beauchemin says she has contacted law enforcement agencies about using the new technology. She is also planning to collect more hair samples and continue her research with a goal of pinpointing where exactly in the world the hair sample is from, to look for more ethnicities and determine specific age.

Source: Queen's University

Read More

Study reveals how dogs detect explosives, offers new training recommendations

A new study found dogs react best to the actual explosive, calling into question the use of products designed to mimic the odor of C-4 for training purposes. Credit: Image courtesy of Indiana University-Purdue University Indianapolis School of Science

A research team at Indiana University-Purdue University Indianapolis (IUPUI) has helped determine the science behind how canines locate explosives such as Composition C-4 (a plastic explosive used by the U.S. military). The study found the dogs react best to the actual explosive, calling into question the use of products designed to mimic the odor of C-4 for training purposes. These findings are the culmination of a four-year contract funded by the U.S. Department of Defense (DOD).

"Appropriately, dogs that are trained to find real explosives are going to find real explosives and not much else," said John Goodpaster, Ph.D., associate professor of chemistry and chemical biology and director for the Forensic and Investigative Sciences Program in the School of Science at IUPUI.

The effectiveness of trained detector dogs is well established, but the study sought to determine which chemical compounds cause a dog to recognize a particular explosive and alert to it. Previous studies have suggested that certain non-explosive chemicals emitted by Composition C-4 cause dogs to alert, and that these specific chemicals could be used as mimic substances to train the dogs in place of real explosives.

In the first phase of the study, IUPUI researchers discovered that the non-explosive chemicals given off by C-4 mimics also are present in a variety of everyday plastic objects. Objects tested included PVC pipes, electrical tape, movie tickets, a plastic grocery bag and plastic food wrapping. Several of the tested items emitted appreciable levels of a mimic compound recommended by some vendors for training canines.

The second phase exposed 33 trained canines from the DOD, Department of Justice, Amtrak and other agencies to these vapors to see if the dogs would respond. The field trials demonstrated that the dogs failed to respond in any significant way to specific odor compounds found in C-4. The results indicate that if the dogs are trained on the full scent, they will only detect real explosives.

"The canines are not easily fooled -- you can't pick and choose components of explosive odors and expect the dog to respond," Goodpaster said. "Dogs are specific and it's the full scent that causes them to alert."

The study also sought to better establish the scientific facts needed for canine detection to be legally admissible evidence -- an effort that found using mimic compounds could present challenges in court. By training with real explosives, false positives are unlikely in the field. Overall, the team recommended that dogs be trained with actual, not mimic, explosives.

While there is technology available to search for explosives, canines remain the best option because of their speed, sensitivity and ability to search large numbers of items, Goodpaster said. Co-authors on the study include current and former IUPUI School of Science undergraduate and graduate students: William Kranz, Kelley Kitts, Nicholas Strange, Joshua Cummins and Erica Lotspeich.

The full study appears in the March 2014 Forensic Science International.

Source: University-Purdue University Indianapolis School of Science

Read More

CT scans could bolster forensic database to ID unidentified remains

Cranium image reconstructed from CT scans. Credit: North Carolina State University

A study from North Carolina State University finds that data from CT scans can be incorporated into a growing forensic database to help determine the ancestry and sex of unidentified remains. The finding may also have clinical applications for craniofacial surgeons.

"As forensic anthropologists, we can map specific coordinates on a skull and use software that we developed -- called 3D-ID -- to compare those three-dimensional coordinates with a database of biological characteristics," says Dr. Ann Ross, a professor of anthropology at NC State and senior author of a paper describing the work. "That comparison can tell us the ancestry and sex of unidentified remains using only the skull -- which is particularly valuable when dealing with incomplete skeletal remains."

However, the size of the 3D-ID database has been limited by the researchers' access to contemporary skulls that have clearly recorded demographic histories.

To develop a more robust database, Ross and her team launched a study to determine whether it was possible to get good skull coordinate data from living people by examining CT scans.

The University of Pennsylvania Museum's Morton Collection provided the NC State researchers with CT scans of 48 skulls. Researchers mapped the coordinates of the actual skulls manually using a digitizer, or electronic stylus. Then they compared the data from the CT scans with the data from the manual mapping of the skulls.

The researchers found that eight bilateral coordinates on the skull -- those found on either side of the head -- were consistent for both the CT scans and manual mapping.

"This will allow us to significantly expand the 3D-ID database," Ross says. "And these bilateral coordinates give important clues to ancestry, because they include cheekbones and other facial characteristics."

However, the five midline coordinates the researchers tested showed inconsistencies between the CT scans and manual mapping. Midline coordinates are those found along the center of the skull, such as the bridge of the nose.

"More research is needed to determine what causes these inconsistencies, and whether we'll be able to retrieve accurate midline data from CT scans," says Amanda Hale, a former master's student at NC State and lead author of the paper.

This research may also help craniofacial surgeons. "An improved understanding of the flaws in how CT scans map skull features could help surgeons more accurately map landmarks for reconstructive surgery," Hale says.

Source: North Carolina State University

Read More

Blazing car murder of 1930 investigated

Slide with Blazing Car victim's sample on it. Sample taken by Sir Bernard Spilsbury.
Credit: University of Leicester

University of Leicester leads collaboration with Northumbria University, Northamptonshire Police and The Royal London Hospital Museum, in investigation of the Blazing Car Murder of 1930

A forensic team from the University of Leicester and Northumbria University has spearheaded an investigation which has shed new light on a murder case from 1930.

A team from the University of Leicester, led by Dr John Bond OBE from the Department of Chemistry and Dr Lisa Smith from the Department of Criminology worked with colleagues from Northumbria University, Northamptonshire Police and The Royal London Hospital Museum to tackle the riddle of the 'Blazing Car Murder' from over 80 years ago.

The case involved the murder of a male in a car fire in Hardingstone, Northamptonshire, on 6 November 1930. Alfred Rouse was convicted, and later hanged, at Bedford Gaol in March 1931, for murdering his victim who to this day, has not been identified.

At the time, a post mortem examination was carried out in the garage of the local public house by the Home Office-appointed pathologist Sir Bernard Spilsbury, working alongside another local pathologist.

Sir Spilsbury reported that lavender coloured material and light brown hair were found at the scene. It was further documented that the victim's jawbone was removed to assist with possible identification and tissue samples taken for microscopical examination.

Two of these tissue samples are still in existence and archived in The Royal London Hospital Museum: one from the prostate to confirm the sex of the victim, and another from the lung to determine whether or not the victim was already dead before the fire was started.

In recent months, attention has turned to the fact that a man named William Briggs left his family home in London to attend a doctor's appointment at around the same time the crime was committed -- and was never seen or heard of again.

As part of their family ancestry research, the relatives of William Briggs wanted to verify earlier generations' belief that their ancestor may have been Rouse's car murder victim.

Last year, a number of William Briggs's relatives approached Northamptonshire Police in an attempt to put the 83-year-old mystery to rest and finally reveal the identity of the victim.

They met with the Force's curator and archivist Richard Cowley, discussed the story of the murder and were shown artefacts relating to the crime which, at the time received worldwide attention.

With the help of Northamptonshire Police, the family contacted University of Leicester academic Dr John Bond OBE. He and Dr Lisa Smith negotiated with The Royal London Hospital museum to allow one of the remaining tissue samples to be examined.

The slide was released with the approval of Professor Richard Trembath, at Queen Mary College University of London. The slide originates from the old Department of Forensic Medicine which formed part of The London Hospital Medical College. The College was merged with Queen Mary College in 1995.

The University of Leicester team considered whether there might just be enough mitochondrial DNA (mtDNA) left on the slide to get a profile to compare with mtDNA from the family.

Mitochondrial DNA is wholly inherited from the maternal line so it is essential to have an unbroken maternal line of descendants to test.

University of Leicester worked with the Northumbria University Centre for Forensic Science and Dr Eleanor Graham, a former member of staff at the University of Leicester, and Victoria Barlow to carry out DNA analysis on the samples to see if there was a match from the sample and the relatives.

Fortunately, the scientists obtained a full single male mtDNA profile from the slide to compare to the family.

Dr John Bond, from the University of Leicester said: "It's been very interesting and rewarding working on such a famous, local murder case. It was quite a unique investigation to be involved in, as the perpetrator had been identified long ago and brought to justice while the victim's identity remained unknown.

"It was a great example of how the scientific and criminological expertise at the University of Leicester and Northumbria University, working together with the police, could provide answers to this family after 83 years."

Detective Chief Superintendent Paul Phillips from Northamptonshire Police said: "From our perspective this is a closed case, the offender Alfred Rouse was convicted of murder and hanged, but this has been a long-standing mystery in Northamptonshire as the identity of the victim has never been established.

"Our work at Northamptonshire Police is victim focused so I was delighted to learn of new opportunities to establish the identity of the victim through the development of forensic science."

Dr Eleanor Graham from Northumbria University stated: "Projects such as this highlight the fact that forensic DNA analysis is not confined to 'catching criminals'. DNA analysis also has a critical role to play in the identification of those who have been killed during criminal acts, accidents or natural disasters, which have occurred recently, or many years ago."

The result is due to be revealed to the family on the BBC's The One Show on a date to be fixed.

Blazing Car Murder background:

Alfred Rouse sustained a head wound in the First World War, which left him with a personality disorder, to the point that he was described as 'a promiscuous rake with an enormous sexual appetite'.

Rouse was a commercial traveller who went all around the country and his promiscuous lifestyle resulted in him facing severe financial problems.

As a consequence, Rouse devised a plan to murder a homeless tramp who would not be missed by anyone which would enable him to stage his own death in a car accident and then disappear to start a new life free from financial restriction.

To that end, Rouse rendered his victim unconscious, placed him in the driver's seat of his car and set the car alight.

Rouse was making his way from the scene but bumped into two local youths keen to see what was going on and take part in some late Bonfire Night celebrations.

This initial contact eventually led to Rouse's arrest. He was convicted at Northampton Assizes and hanged in Bedford on 10 March 1931.

The local Herald newspaper suggested that the identity of Rouse's victim 'would likely remain a mystery forever.' But will it………..?

Source: University of Leicester

Read More